Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Denk, H; Lackinger, E; Zatloukal, K; Franke, WW.
Turnover of cytokeratin polypeptides in mouse hepatocytes.
Exp Cell Res. 1987; 173(1):137-143
Doi: 10.1016/0014-4827(87)90339-9
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- Führende Autor*innen der Med Uni Graz
- Denk Helmut
- Co-Autor*innen der Med Uni Graz
- Zatloukal Kurt
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- Abstract:
- The turnover of cytokeratin polypeptides A (equivalent to No. 8 of the human cytokeratin catalog) and D (equivalent to human cytokeratin No. 18) of mouse hepatocytes was studied by pulse-labeling of mouse liver proteins after intraperitoneal injection of L-[guanido-14C]arginine and [14C]sodium bicarbonate. At various times after injection cytoskeletal proteins were prepared and separated by SDS-polyacrylamide gel electrophoresis, and the specific radioactivities of polypeptides recovered from excised gel slices were determined. With L-[guanido-14C]arginine a rapid increase in the specific radioactivity of both cytokeratins was observed which reached a plateau between 12 and 24 h. With [14C]sodium bicarbonate maximal specific radioactivity was obtained at 6 h followed by a rapid decrease to half maximum values within the subsequent 6 h and then a slower decrease. Half-lives were determined from the decrease of specific radioactivities after pulse-labeling by least-squares plots and found to be 84 h (for cytokeratin component A) and 104 h (component D) for arginine labeling. Values obtained after bicarbonate labeling were similar (95 h for A and 98 h for D). These results show that liver cytokeratins are relatively stable proteins and suggest that components A and D are synthesized and degraded at similar rates, probably in a coordinate way.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Arginine - diagnostic use
- Bicarbonates - metabolism
- Cytoskeleton - metabolism
- Intermediate Filaments - metabolism
- Keratins - metabolism
- Liver - metabolism
- Mice - metabolism