Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
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SHR Neuro Krebs Kardio Lipid Stoffw Microb
Saxena, AK; Kofler, K; Ainödhofer, H; Höllwarth, ME.
Esophagus tissue engineering: hybrid approach with esophageal epithelium and unidirectional smooth muscle tissue component generation in vitro.
J Gastrointest Surg. 2009; 13(6):1037-1043
Doi: 10.1007/s11605-009-0836-4
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- Führende Autor*innen der Med Uni Graz
- Saxena Amulya Kumar
- Co-Autor*innen der Med Uni Graz
- Ainödhofer Herwig
- Höllwarth Michael
- Kofler Kristina
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- Abstract:
- The aim of this study was to engineer the two main components of the esophagus in vitro: (a) esophageal epithelium and (b) smooth muscle tissue. Furthermore, (a) survivability of esophageal epithelial cells (EEC) on basement membrane matrix (BMM)-coated scaffolds and (b) oriented smooth muscle tissue formation on unidirectional BMM-coated collagen scaffolds was investigated. Both EEC and smooth muscle cells (SMC) were sourced from Sprague-Dawley rats. The EEC were maintained in vitro and seeded onto BMM-coated 2-D collagen scaffolds. Similarly, smooth muscle cells were obtained using an explants technique and seeded on unidirectional 3-D BMM-coated collagen scaffolds. Cell-polymer constructs for EEC and SMC were maintained in vitro for 8 weeks. Protocols to obtain higher yield of EEC were established. EEC formed a layer of differentiated epithelium after 14 days. EEC survivability on polymers was observed up to 8 weeks. Unidirectional smooth muscle tissue strands were successfully engineered. Esophageal epithelium generation, survivability of EEC on BMM-coated scaffolds, and engineering of unidirectional smooth muscle strands were successful in vitro. The hybrid approach of assembling individual tissue components in vitro using BMM-coated scaffolds and later amalgamating them to form composite tissue holds promises in the tissue engineering of complex organ systems.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Culture Techniques -
- Cells, Cultured -
- Collagen -
- Esophagus - cytology
- Muscle, Smooth - cytology
- Polymers -
- Rats -
- Rats, Sprague-Dawley -
- Tissue Engineering - methods
- Tissue Scaffolds -
- Find related publications in this database (Keywords)
- Tissue engineering
- Esophagus
- Epithelium
- Smooth muscle
- Collagen
- Hybrid
- Basement membrane matrix