Medizinische Universität Graz - Research portal

Go directly to the nagivation.
Go directly to the content.

Navigation/Search

Selected Publication:

SHR Neuro Cancer Cardio Lipid Metab Microb

Rosker, C; Meur, G; Taylor, EJ; Taylor, CW.
Functional Ryanodine Receptors in the Plasma Membrane of RINm5F Pancreatic {beta}-Cells.
J Biol Chem. 2009; 284(8): 5186-5194. Doi: 10.1074/jbc.M805587200 [OPEN ACCESS]
Web of Science PubMed FullText FullText_MUG

Leading authors Med Uni Graz: Rosker Christian
Altmetrics:
Dimensions Citations:
Plum Analytics:
Scite (citation analytics):
Abstract:: Ryanodine receptors (RyR) are Ca(2+) channels that mediate Ca(2+) release from intracellular stores in response to diverse intracellular signals. In RINm5F insulinoma cells, caffeine, and 4-chloro-m-cresol (4CmC), agonists of RyR, stimulated Ca(2+) entry that was independent of store-operated Ca(2+) entry, and blocked by prior incubation with a concentration of ryanodine that inactivates RyR. Patch-clamp recording identified small numbers of large-conductance (gamma(K) = 169 pS) cation channels that were activated by caffeine, 4CmC or low concentrations of ryanodine. Similar channels were detected in rat pancreatic beta-cells. In RINm5F cells, the channels were blocked by cytosolic, but not extracellular, ruthenium red. Subcellular fractionation showed that type 3 IP(3) receptors (IP(3)R3) were expressed predominantly in endoplasmic reticulum, whereas RyR2 were present also in plasma membrane fractions. Using RNAi selectively to reduce expression of RyR1, RyR2, or IP(3)R3, we showed that RyR2 mediates both the Ca(2+) entry and the plasma membrane currents evoked by agonists of RyR. We conclude that small numbers of RyR2 are selectively expressed in the plasma membrane of RINm5F pancreatic beta-cells, where they mediate Ca(2+) entry.
Find related publications in this database (using NLM MeSH Indexing): Animals -; Caffeine - pharmacokinetics; Calcium - metabolism; Calcium Channel Agonists - pharmacology; Cell Line, Tumor -; Cell Membrane - metabolism; Central Nervous System Stimulants - pharmacology; Cresols - pharmacology; Dose-Response Relationship, Drug -; Fungicides, Industrial - pharmacology; Inositol 1,4,5-Trisphosphate Receptors - agonists; Insulin-Secreting Cells - metabolism; Male -; Rats -; Rats, Wistar -; Ryanodine - pharmacology; Ryanodine Receptor Calcium Release Channel - biosynthesis