Medizinische Universität Graz - Research portal
Selected Publication:
Heiser, M; Hutter-Paier, B; Jerkovic, L; Pfragner, R; Windisch, M; Becker-Andre, M; Dieplinger, H.
Vitamin E binding protein Afamin protects neuronal cells in vitro
. 2002; 337-345.
Web of Science
- Co-authors Med Uni Graz
- Pfragner Roswitha
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- Abstract:
- Afamin, an 87kDa human plasma glycoprotein with specific binding properties for vitamin E (alpha-tocopherol) was recently characterized (Jerkovic, 1997; Vogele, 1999). In the present study the in vitro effects on neuronal cells of native human Afamin, of Afamin pre-loaded with vitamin E (Afamin+), and of vitamin E were investigated. Isolated cortical chicken neurons were maintained either under apoptosis-inducing low serum conditions or exposed to oxidative stress by the addition of H2O2 or beta-amyloid peptide(25-35). Afamin and vitamin E synergistically enhance the survival of cortical neurons under apoptotic conditions. Furthermore, Afamin alone protects cortical neurons from cell death in both experimental settings. Therefore, the plasma glycoprotein Afamin apparently displays a neuroprotective activity not only by virtue of binding and transporting vitamin E but also on its own.