Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Leis, HJ; Malle, E; Mayer, B; Kostner, GM; Esterbauer, H; Gleispach, H.
Quantitative measurement of 5-, 12-, and 15-hydroxyeicosatetraenoic acid together with 12-hydroxyheptadecatrienoic acid by stable isotope dilution gas chromatography-negative ion chemical ionization-mass spectrometry.
Anal Biochem. 1987; 162(2):337-344
Doi: 10.1016/0003-2697(87)90401-5
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- Führende Autor*innen der Med Uni Graz
- Leis Hans-Joerg
- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
- Malle Ernst
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- Abstract:
- A stable isotope dilution gas chromatography-negative ion chemical ionization-mass spectrometry assay for simultaneous quantitative measurement of 5-hydroxyeicosatetraenoic acid (HETE), 12-HETE, 15-HETE, and 12-hydroxyheptadecatrienoic acid in one single GC/MS run was established. 18O2-Labeled analogs as internal standards, prepared according to conventional procedures, were found to be useful for this application. A sample processing and derivatization sequence providing highly purified compounds with a recovery of 42.7% was elaborated. The detection limit was in the femtomole range.
- Find related publications in this database (using NLM MeSH Indexing)
- 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid -
- Chromatography, High Pressure Liquid -
- Chromatography, Thin Layer -
- Fatty Acids, Unsaturated - analysis
- Gas Chromatography-Mass Spectrometry - methods
- Hydroxyeicosatetraenoic Acids - analysis
- Indicator Dilution Techniques - analysis
- Isotope Labeling - analysis
- Oxygen Isotopes - analysis