Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Gries, A; Nimpf, J; Nimpf, M; Wurm, H; Kostner, GM.
Free and Apo B-associated Lpa-specific protein in human serum.
Clin Chim Acta. 1987; 164(1):93-100
Doi: 10.1016/0009-8981(87)90110-0
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- Führende Autor*innen der Med Uni Graz
- Gries Anna
- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
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- Abstract:
- The distribution of Lpa-specific protein (Apo A) among various fractions of human serum was investigated. The following results were obtained. In very low density lipoproteins (VLDL) of fasting human serum Apo A cannot be detected. Most of Apo A is complexed to LDL forming lipoprotein a (Lpa) present in the density fraction of 1.006-1.125 g/ml. Only approximately 5% of Apo A are found in the d greater than 1.125 bottom fraction after ultracentrifugation. Phosphotungstate-Mg (PTA), heparin-Mn (Hep) and dextran sulfate-Mg (DS) precipitate approximately 95% of serum Lpa together with the Apo B containing lipoproteins. Also monospecific antibodies against Apo B do not precipitate all Lpa and leave approximately 3% of Apo A in the supernatant. With polyethylene glycol (PEG) on the other hand, virtually all Lpa is removed since this reagent also precipitates Lpa and Apo A. Apo A of the bottom fraction after ultracentrifugation and of the supernatant after selective precipitation is not stainable with Sudan black and probably represents a protein which is not associated with lipids.
- Find related publications in this database (using NLM MeSH Indexing)
- Adolescent -
- Adult -
- Apolipoproteins A - blood
- Female - blood
- Humans - blood
- Immunochemistry - blood
- Lipoprotein(a) - blood
- Lipoproteins - blood
- Male - blood
- Precipitation - blood
- Ultracentrifugation - blood