Medizinische Universität Graz - Research portal
Selected Publication:
Schäfer, U; Seibold, S; Schneider, A; Neugebauer, E.
Isolation and characterisation of the human rab18 gene after stimulation of endothelial cells with histamine.
FEBS Lett. 2000; 466(1): 148-154.
Doi: 10.1016/S0014-5793(99)01778-0
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- Leading authors Med Uni Graz
- Schäfer Ute
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- Abstract:
- Here we report the isolation of a cDNA encoding the complete human rab18 protein from histamine-stimulated endothelial cells using differential display. The amino acid sequence showed 98% homology with the previously isolated mouse rab18 protein, which is implicated in apical/basolateral endocytosis. Northern blot analysis revealed two transcripts (2.5 kb and 1 kb) ubiquitously expressed in all examined organs, as well as in human umbilical vein endothelial and aortic cells. In blood cells rab18 transcripts were hardly detectable. The histamine-induced time-dependent increase of rab18 mRNA expression in human umbilical vein endothelial cells and peripheral blood mononuclear cells indicates for the first time a link between receptor-mediated signal transduction and the regulation of rab gene expression. This finding might also imply a role for rab proteins in inflammation.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acid Sequence -
- Animals -
- Base Sequence -
- Cells, Cultured -
- Cloning, Molecular -
- DNA Primers - genetics
- DNA, Complementary - genetics
- Endothelium, Vascular - drug effects
- Female - drug effects
- Gene Expression Regulation - drug effects
- Histamine - pharmacology
- Humans - pharmacology
- Leukocytes, Mononuclear - drug effects
- Male - drug effects
- Mice - drug effects
- Molecular Sequence Data - drug effects
- RNA, Messenger - genetics
- Sequence Homology, Amino Acid - genetics
- Signal Transduction - genetics
- rab GTP-Binding Proteins - genetics
- Find related publications in this database (Keywords)
- GTP-binding protein
- histamine
- lymphocyte
- human umbilical vein endothelial cell
- regulation of gene expression