Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Gasser, S; Ablasser, K; Roessl, U; Dellacher, A; von Lewinski, D; Mangge, H; Machler, H; Trantina, A; Tscheliessnigg, KH; Udermann, H; Porta, S; Friehs, I; Scherr, E; Gasser, R.
A microarray study on the effect of extracellular magnesium deprivation upon expression profiles of molecular pathways and biological processes in isolated human atrial myocardium
TRACE ELEM ELECTROLYTES. 2009; 26(1): 1-14.
Doi: 10.5414/TEP26001
Web of Science
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- Führende Autor*innen der Med Uni Graz
- Gasser Robert
- Gasser Sandra Renate
- Co-Autor*innen der Med Uni Graz
- Ablasser Klemens
- Mangge Harald
- Porta Sepp
- Rössl Ulrich
- Scherr Elisabeth
- Tscheliessnigg Karlheinz
- von Lewinski Dirk
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- Abstract:
- While we well understand that nutritional Mg2+-deprivation is lethal and caused numerous cellular dysfunctions ending up with myocardial cell necrosis, the effects of extracellular Mg2+-concentrations on molecular pathways and processes has not been studied as yet. Characterizing these Mg2+-dependent intracellular molecular pathways may well constitute a further step towards understanding the effects of magnesium on myocardial function as well as protection. Using molecular profiling technique, we look at over 20,000 different gene expressions in the presence and absence of extracellular M-g2+, thus identifying the specific molecular signature of myocardial Mg2+-deprivation. This allows LIS to demonstrate its effects at the molecular level in resting human atrial myocardium. Using PANTHER software (Applied Biosystems) we assess up- and down-regulation of gene expression associated with biological processes and pathways. Myocardial gene expression after exposure of 30 minutes to Mg2+-free solution is massively altered compared to control experiments. We find a complex de-regulation of gene expression secondary to Mg2+ deficiency. It can be seen that gene expression associated with clusters of immunity and defence processes' protein metabolism and signal transduction as well as nucleoside, nucleotide and nucleic metabolism are significantly down-regulated. Similarly, biological processes involved in transcription, protein biosynthesis and cell communication, nucleoside, nucleotide and nucleic metabolism as well as signal transduction and protein metabolism are effected by Up-regulation. Clusters of pathways down-regulated by Mg2+ deficiency are: various signaling pathways, T-cell activation, apoptosis and angiogenesis. Clusters of up-regulated pathways are mainly different groups of signaling. In summary, experimental myocardial Mg2+-deprivation leads to complex changes in the expression profile of biological processes and molecular pathways. On the other hand, one can deduce from earlier observations that Mg2+, is well buffered and remains relatively uninfluenced by extracellular maneuvers. The interdependence of intracellular and extracellular Mg2+, however, appears once more difficult to understand. Further studies are certainly needed in order to firmly establish the mechanisms of extracellular Mg2+ influencing intracellular processes and pathways.
- Find related publications in this database (Keywords)
- magnesium
- myocardial
- molecular pathways
- biological processes