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Selected Publication:
Hoffmann, O; Klaushofer, K; Gleispach, H; Leis, HJ; Luger, T; Koller, K; Peterlik, M.
Gamma interferon inhibits basal and interleukin 1-induced prostaglandin production and bone resorption in neonatal mouse calvaria.
Biochem Biophys Res Commun. 1987; 143(1):38-43
Doi: 10.1016/0006-291X(87)90626-7
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- Co-authors Med Uni Graz
- Leis Hans-Joerg
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- Abstract:
- Production of the osteolytic arachidonic acid metabolites, prostaglandin (PG) E2, PGI2 and PGF2 alpha, by neonatal mouse calvariae was quantitated by gas chromatography/mass spectrometry. Mouse recombinant interleukin 1 (rIL-1) raised medium levels of PGE2 and PGI2 (measured as 6-keto-PGF1 alpha) in the dose range tested (1.0-10.0 U/ml culture medium), while an effect on PGF2 was only observed at 10 U/ml. Bone resorption in response to rIL-1 reached a plateau at 3.0 U/ml. Mouse recombinant gamma-interferon (rIFN-gamma) between 100-500 U/ml suppressed basal PG synthesis and spontaneous resorption of cultured bone. In addition, IFN-gamma at 100 U/ml prevented stimulation of PG synthesis by 3.0 U/ml rIL-1 and thereby reduced the bone resorbing activity of the cytokine by at least 60%. 5 X 10(-7) M indomethacin was equally effective in suppression of PG synthesis and bone resorption. The present study provides evidence that IFN-gamma inhibits PG synthesis and consequently resorption of cultured bone.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Animals, Newborn -
- Bone Resorption - drug effects
- Bone and Bones - drug effects
- Indomethacin - pharmacology
- Interferon Type II - pharmacology
- Interleukin-1 - physiology
- Mice - physiology
- Mice, Inbred Strains - physiology
- Organ Culture Techniques - physiology
- Prostaglandins - biosynthesis
- Recombinant Proteins - pharmacology