Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
PALLAPIES, D; BURCHERT, M; PESKAR, BA; DEMBINSKAKIEC, A; PESKAR, BM.
EFFECT OF 5-AMINOSALICYLIC ACID AND 4-AMINOSALICYLIC ACID ON PRECONTRACTED RAT AORTIC STRIPS AND ON NO-MEDIATED INHIBITION OF PLATELET-AGGREGATION
AGENTS AND ACTIONS; 41: C235-C237.
Doi: 10.1007/BF01987651
Web of Science
FullText
FullText_MUG
Google Scholar
- Co-Autor*innen der Med Uni Graz
- Peskar Bernhard
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- We have examined the interactions of 5-aminosalicylic acid (5-ASA) and 4-aminosalicylic acid (4-ASA) with nitric oxide (NO) on rat aorta and human platelets. Phenylephrine-precontracted rat aortic strips with intact endothelium were further contracted by 5-ASA (50-200 mu mol/l) and 4-ASA (1-20 mmol/l) in a concentration-dependent manner. Removal of endothelium, inhibition of guanylate cyclase by methylene blue, inhibition of NO biosynthesis by N-G-nitro-L-arginine as well as inactivation of NO by oxyhemoglobin abolished the effects of 5-ASA and 4-ASA. The antiaggregatory effects of 3-morpholinosydnonimine (SIN-1) and rat peritoneal neutrophils (RPN) were diminished in a concentration-dependent manner by 5-ASA (50-250 mu mol/l), but not by 4-ASA (up to 20 mmol/l). In the presence of superoxide dismutase (SOD), 5-ASA did not antagonize NO-mediated effects on platelets. 5-ASA up to 100 mu mol/l did not affect NO synthase from rat brain, while a concentration of 1 mM caused 21% inhibition. Since NO might act as a cytotoxic mediator, our results suggest that inactivation of NO by 5-ASA and higher concentrations of 4-ASA could contribute to the therapeutic activity of the drugs in inflammatory bowel disease (IBD).