Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Sedej, S; Gurung, IS; Binz, T; Rupnik, M.
Phosphatidylinositol-4,5-bisphosphate-dependent Facilitation of the ATP-dependent Secretory Activity in Mouse Pituitary Cells.
Ann N Y Acad Sci. 2009; 1152(1): 165-173.
Doi: 10.1111/j.1749-6632.2008.04002.x
Web of Science
PubMed
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- Leading authors Med Uni Graz
- Sedej Simon
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- Abstract:
- Phosphatidylinositol-4, 5-bisphosphate [PI(4,5)P(2)] has been implicated in the priming of large dense-core vesicles in many secretory cells; however, its role in the Ca(2+)-dependent secretory activity in pituitary cells remains elusive. We assessed the effect of elevated intracellular PI(4,5)P(2) on the kinetics of Ca(2+)-dependent exocytosis, using a whole-cell patch-clamp technique in wild-type mouse melanotrophs from fresh pituitary tissue slices. We found that 1 micromol/L PI(4,5)P(2) significantly increased Ca(2+)-dependent exocytosis of vesicles that need to go through ATP-dependent reactions; however, the exocytosis of release-ready vesicles (ATP-independent release) and voltage-activated Ca(2+) currents remained unaffected. We suggest that PI(4,5)P(2) increases the size of the readily releasable vesicle pool by regulating the effectiveness of vesicular mobilization and fusion in an ATP-dependent manner.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenosine Triphosphate - metabolism
- Animals -
- Calcium - metabolism
- Electrophysiology -
- Female -
- Male -
- Mice -
- Patch-Clamp Techniques -
- Phosphatidylinositol 4,5-Diphosphate - metabolism
- Pituitary Gland - metabolism
- Find related publications in this database (Keywords)
- phosphatidylinositol-4
- 5-biphosphate
- release-ready vesicles
- Ca2+-dependent exocytosis
- priming
- capacitance measurements
- ATP-independent pathway
- ATP-dependent pathway
- acute tissue slices
- pituitary
- melanotrophs