Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Geigl, J; Speicher, M.
New methods of single-cell analysis in research and diagnostics
MED GENET. 2008; 20(4): 407-415.
Doi: 10.1007/s11825-008-0138-3
Web of Science
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Geigl Jochen Bernd
- Co-Autor*innen der Med Uni Graz
- Speicher Michael
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Traditional cytogenetics is a paradigm for single-cell diagnostics; after a banding procedure, each metaphase examined represents the analysis of an entire genome of a cell, albeit at a low resolution. For several decades, this single-cell character has represented an important distinction in molecular genetics technologies, which are mostly based on DNA or RNA extracted from hundreds or thousands of cells. However, many essential questions can be addressed only by analyzing cells on the level of fewer or single cells. In the last few years, new single-cell techniques have been developed with the aim to simultaneously examine more regions with improved resolution. In this overview we summarize the most important recent developments and changes.
- Find related publications in this database (Keywords)
- Single-cell analysis
- Unbiased whole-genome amplification
- Comparative genomic hybridization (CGH)
- Copy number variation (CNV)
- Resolution limits