Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
FOLDESPAPP, Z; BIRCHHIRSCHFELD, E; ROSCH, R; HARTMANN, M; KLEINSCHMIDT, AK; SELIGER, H.
EFFICIENCY OF CHEMICAL OLIGONUCLEOTIDE SYNTHESIS EVALUATED BY ION-EXCHANGE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY
J CHROMATOGR A. JOURNAL OF CHROMATOGRAPHY A; 706: 405-419. ( Presented at: 7th International Ion Chromatography Symposium (IICS 94), TURIN, ITALY, SEP 19-22, 1994)
Doi: 10.1016/0021-9673(94)01221-Y
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- Földes-Papp Zeno
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- Abstract:
- Ion-exchange high-performance liquid chromatography (HPIEC) is a method of choice for the separation of crude solid-phase synthesized oligonucleotides. It shows peaks for target and error sequences and it is used for quantification of oligonucleotide synthesis. This paper presents a homeodynamic model of polymer-supported oligonucleotide synthesis and a numerical solution, which accounts for elution profiles obtained by HPIEC. The elution profiles are consistent with the model. The chain-length distributions for 30mer syntheses on different support materials were also studied and the probability moment analysis was applied as a quantitative measure of the differential relationship of fractal dimension. The support systems for chemical synthesis of short oligonucleotides are classified according to this relationship.