Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Gosch, M; Foldes-Papp, Z; Blom, H; Holm, J; Heino, T; Thyberg, P; Bjork, G; Rigler, R.
Single molecule detection and bead trapping in microstructures for molecule selection and DNA degradation
MESA MG. 2000; 427-430.
Web of Science
- Co-Autor*innen der Med Uni Graz
- Földes-Papp Zeno
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- Abstract:
- Single diffusing, as well as fast flowing (10 mm/s), tetramethylrhodamine labeled molecules (TMR-4-dUTP) were detected in silicon microstructures by confocal fluorescence microscopy. Low fluorescent silicon-glass microstructures, manufactured by Deep Reactive Ion Etching (DRIE), enabled us to obtain a signal-to-noise ratio of 10:1 for diffusion and 4:1 for 10 mm/s flow. Further, laser bead trapping of 1 mum silica beads with a Nd:YAG laser has been performed. A laser power of 55 mW was sufficient to hold the beads in a 10 mm/s laminar flow.
- Find related publications in this database (Keywords)
- single molecule
- FCS
- bead trapping
- hydrodynamic flow
- microstructures