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Zechner, R; Moser, R; Kostner, GM.
Isolation of pure LpB from human serum.
J Lipid Res. 1986; 27(6):681-686 Doi: 10.1016/S0022-2275(20)38807-6
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Co-Autor*innen der Med Uni Graz: Kostner Gerhard
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Abstract:: Low density lipoproteins (LDL), even after isolation from a narrow density cut and after several washes by preparative ultracentrifugation, are contaminated by 3-5% non-apoB proteins. Incubation of these LDL with artificial triglyceride-rich lipid emulsions (TGRP) removed all contaminating apoC and also, under certain conditions, apoA proteins. TGRP treatment did not, however, change the lipid composition and the flotation behavior of LDL. Residual apoE and albumin, amounting up to 0.5% of the apoB mass, were resistant to removal by TGRP treatment as well as by heparin-Sepharose column chromatography. ApoE and albumin could only be removed by immunoabsorption.
Find related publications in this database (using NLM MeSH Indexing): Apolipoproteins - blood; Apolipoproteins B - blood; Emulsions - blood; Humans - blood; Immunosorbent Techniques - blood; Lipoproteins, LDL - blood; Serum Albumin - isolation and purification; Triglycerides - isolation and purification; Ultracentrifugation - isolation and purification