Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Hesz, A; Ingolic, E; Krempler, F; Kostner, GM.
The existence of B/E and E receptors on Hep-G2 cells: a study using colloidal gold- and 125I-labeled lipoproteins.
Exp Mol Pathol. 1987; 46(3):372-382
Doi: 10.1016/0014-4800(87)90057-8
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- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
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- Abstract:
- The presence of specific receptors for apolipoprotein B (low-density lipoproteins) and apolipoprotein E (HDL-E) on Hep-G2 cells and human skin fibroblasts was studied by chemical methods and by electron microscopy using a differential gold labeling technique. Fibroblasts bound both types of lipoproteins to one and the same receptor (B/E receptor) as deduced from competition experiments with HDL-E and LDL. Labeled HDL-E, on the other hand, was only partially displaced by cold LDL but was completely displaced by unlabeled HDL-E. Scatchard analysis of lipoprotein binding to Hep-G2 cells revealed an approx 10 times higher binding affinity of apoE-containing lipoproteins as compared to apoB-containing ones. No differences between apoE- or apoB-containing lipoproteins with respect to the morphology of cell binding and intracellular processing were observed. The results are compatible with the concept that Hep-G2 cells possess two kinds of receptors, one specific for apoB- and apoE-containing lipoproteins (B/E receptor) and another specific for apoE only. From these studies we conclude that Hep-G2 cells may serve as a suitable model for studying the lipoprotein metabolism in the liver.
- Find related publications in this database (using NLM MeSH Indexing)
- Apolipoproteins B - metabolism
- Apolipoproteins E - metabolism
- Binding, Competitive - metabolism
- Cell Line - metabolism
- Fibroblasts - metabolism
- Gold Colloid, Radioactive - diagnostic use
- Humans - diagnostic use
- Iodine Radioisotopes - diagnostic use
- LDL-Receptor Related Protein 1 - diagnostic use
- Liver - metabolism
- Receptors, Cell Surface - metabolism
- Receptors, Lipoprotein - metabolism