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Mombouli, JV; Zeldin, D; Scott-Burden, T; Holzmann, S; Kostner, GM; Graier, WF.
Epoxyeicosatrienoic acids potentiate Ca2+ signaling in both endothelial and vascular smooth muscle cells
. 1999; 77-83.
Web of Science

Co-authors Med Uni Graz: Graier Wolfgang; Kostner Gerhard
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Abstract:: Experiments were designed to assess the impact on Ca2+ signaling of sustained increases in the concentration epoxyeicosatrienoic acids (EETs) in cultured endothelial or vascular smooth muscle cells. Cultured human umbilical vein-derived endothelial cells (EA.hy926) and bovine aortic vascular smooth muscle cells were processed for conventional spectrofluorometric measurement of cytosolic Ca2+, using Fura2/AM. A bovine aortic vascular smooth muscle cell line expressing the CYP2J2 epoxygenase was developed to determine the impact of chronic enhancement of epoxygenase activity. In EA.hy926 endothelial cells, the histamine-induced mobilization of Ca2+ from intracellular stores, as well as the stimulation of Ca2+ influx were inhibited by the cytochrome P450 inhibitor thiopental. However, incubation of the cells with 11,12-EET amplified the mobilization of Ca2+ from both intracellular and extracellular pools. In this case, thiopental no longer affects significantly the mobilization of Ca2+ induced by histamine in EA.hy926 cells. A similar potentiation of Ca2+ signaling was obtained in bovine aortic vascular smooth muscle cells stimulated with thapsigargin. In vascular smooth muscle cells overexpressing the epoxygenase CYP2J2, an increased spontaneous Ca2+ influx was obtained, as well as an amplification of endothelin 1-induced capacitative Ca2+ entry. These results show that sustained increases in EET levels result in an amplification of the mobilization of cytosolic Ca2+ in cultured endothelial and vascular smooth muscle cells.
Find related publications in this database (Keywords): Cytochrome P450; capacitative Ca2+ entry; arachidonic acid; thapsigargin; endothelin