Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Malle, E; Leonhard, B; Knipping, G; Sattler, W.
Effects of cytokines, butyrate and dexamethasone on serum amyloid A and apolipoprotein A-I synthesis in human HUH-7 hepatoma cells.
Scand J Immunol. 1999; 50(2):183-187
Doi: 10.1046%2Fj.1365-3083.1999.00574.x
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- Führende Autor*innen der Med Uni Graz
- Malle Ernst
- Co-Autor*innen der Med Uni Graz
- Sattler Wolfgang
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- Abstract:
- Serum amyloid A (SAA) and apolipoprotein A-I (apo A-I) are secreted by the liver. As concentrations of both apolipoproteins are inversely related under normal and acute-phase conditions, human HUH-7 hepatoma cells were stimulated with interleukin (IL)-1alpha (100 and 200 U), IL-6 (50 and 100 U), butyrate (2 mM) and dexamethasone (2 x 10(-7)M and 1 x 10(-6)M), alone or in combination. Changes in SAA and apo A-I synthesis were monitored after metabolic labelling of the cells with [35S]-methionine. Intracellular and secreted SAA and apo A-I were immunoprecipitated, separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), and the radioactivity in the corresponding bands was counted. Intracellular apolipoprotein levels were increased by all stimuli, either alone or in combination, between 2.7- and 5.5-fold (SAA) and between 2.8- and 4.1-fold (apo A-I), respectively. In a similar manner, apolipoprotein levels secreted by HUH-7 cells were increased between 3.1- and 4.3-fold (SAA) and between 1.9- and 3. 3-fold (apo A-I). Co-administration of cytokines, butyrate and/or dexamethasone had no pronounced synergistic effect on intracellular biosynthesis and secretion of SAA and apo A-I. The results from the present study suggest that apo A-I must not necessarily be considered as a negative acute-phase reactant.
- Find related publications in this database (using NLM MeSH Indexing)
- Acute-Phase Reaction - immunology
- Apolipoprotein A-I - biosynthesis
- Apolipoproteins - biosynthesis
- Butyrates - immunology
- Carcinoma, Hepatocellular - immunology
- Dexamethasone - immunology
- Glucocorticoids - immunology
- Humans - immunology
- Interleukin-1 - immunology
- Interleukin-6 - immunology
- Serum Amyloid A Protein - biosynthesis
- Tumor Cells, Cultured - biosynthesis