Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Behrends, M; Schulz, R; Post, H; Alexandrov, A; Belosjorow, S; Michel, MC; Heusch, G.
Inconsistent relation of MAPK activation to infarct size reduction by ischemic preconditioning in pigs.
Am J Physiol Heart Circ Physiol. 2000; 279(3):H1111-H1119
Doi: 10.1152/ajpheart.2000.279.3.H1111
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- Co-Autor*innen der Med Uni Graz
- Post Heiner
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- Abstract:
- The importance of the activation of mitogen-activated protein kinases (MAPK) for the cardioprotection achieved by ischemic preconditioning (IP) is still controversial. We therefore measured infarct size and p38, extracellular signal-regulated kinase (ERK), and c-Jun NH(2)-terminal kinase (JNK) MAPK phosphorylation (by biopsies) in enflurane-anesthetized pigs. After 90 min low-flow ischemia and 120 min reperfusion, infarct size averaged 18.3 +/- 12.4 (SD)% (group 1, n = 14). At similar subendocardial blood flows, IP by 10 min ischemia and 15 min reperfusion (group 2, n = 14) reduced infarct size to 6.2 +/- 5.1% (P < 0.05). An inconsistent increase in p38, ERK, and p54 JNK phosphorylation (by Western blot) was found during IP; p46 JNK phosphorylation increased with the subsequent reperfusion. At 8 min of the sustained ischemia, p38, ERK, and p54 JNK phosphorylation were increased with no difference between groups (medians: p38: 207% of baseline in group 1 vs. 153% in group 2; ERK: 142 vs. 144%; p54 JNK: 171 vs. 155%, respectively). MAPK phosphorylation and reduction of infarct size by IP were not correlated, thus not supporting the concept of a causal role of MAPK in mediating cardioprotection by IP.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Coronary Circulation -
- Disease Models, Animal -
- Female -
- Hemodynamics -
- Ischemic Preconditioning, Myocardial -
- JNK Mitogen-Activated Protein Kinases -
- Male -
- Mitogen-Activated Protein Kinases - metabolism
- Myocardial Infarction - metabolism
- Myocardium - metabolism
- Phosphorylation - metabolism
- Swine, Miniature - metabolism
- p38 Mitogen-Activated Protein Kinases - metabolism
- Find related publications in this database (Keywords)
- p38
- extracellular signal-regulated kinase
- c-Jun NH2-terminal kinase
- Western blot
- myocardial ischemia-reperfusion