Medizinische Universität Graz - Research portal
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Pabst, MA.
Staining of different endocrine cells with hydrochloric acid-toluidine blue in Epon embedded rat tissue.
Stain Technol. 1985; 60(2):93-98
Doi: 10.3109/10520298509113898
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- Pabst Maria-Anna
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- Abstract:
- The usual HCl-toluidine blue staining of different endocrine cells is applicable to paraffin embedded material. A modification for Epon embedded tissue suitable for consecutive light and electron microscopic studies is described which makes it possible to find the same stained cell, both in a semithin section and in subsequent ultrathin sections. This method facilitates the search for scattered specific endocrine cells. Without removing the resin, sections of Epon embedded tissues were hydrolyzed for 17 hr in 1% HCl at 65 C and stained for 2 hr in 0.1% toluidine blue in McIlvaine buffer, pH 5.8. The following cells were stained: C cells in thyroid glands; A and D cells in pancreatic islets; B cells in anterior pituitary; G, D and Ec cells in the gastrointestinal tract; Ad cells of the adrenal medulla.
- Find related publications in this database (using NLM MeSH Indexing)
- Adrenal Medulla - cytology
- Animals - cytology
- Endocrine Glands - cytology
- Epoxy Resins - cytology
- Hydrochloric Acid - diagnostic use
- Islets of Langerhans - cytology
- Jejunum - cytology
- Microscopy, Electron - cytology
- Microtomy - methods
- Pituitary Gland, Anterior - cytology
- Pylorus - cytology
- Rats - cytology
- Rats, Inbred Strains - cytology
- Staining and Labeling - methods
- Thyroid Gland - cytology
- Tolonium Chloride - diagnostic use