Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Knipping, G; Steyrer, E; Holasek, A.
Chromatofocusing of apolipoproteins from human serum high density lipoprotein.
INT J BIOCHEM. 1984; 16: 1149-1154.
Doi: 10.1016/0020-711X(84)90008-9
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- Co-Autor*innen der Med Uni Graz
- Steyrer Ernst
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- Abstract:
- Human HDL was delipidated and the apolipoproteins were fractionated by chromatofocusing. Chromatofocusing, which separates proteins due to their differing isoelectric points, resulted in 8 peaks with corresponding pI values of 7.40, 6.92, 6.64, 5.48, 5.30, 5.18, 4.92 and 4.63. By one single chromatofocusing run four apolipoproteins were obtained in pure form. Two additional polypeptides could be purified during the desalting step using phenyl-Sepharose.
- Find related publications in this database (using NLM MeSH Indexing)
- Apolipoproteins - analysis
- Chromatography, Agarose - analysis
- Electrophoresis, Polyacrylamide Gel - analysis
- Humans - analysis
- Isoelectric Focusing - analysis
- Lipoproteins, HDL - analysis
- Male - analysis