Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Muntean, W; Rothwangl, HJ.
Dissociation of the Factor-VIII complex during clotting: role of thrombin and phospholipids.
Eur J Clin Invest. 1984; 14(6):462-468
Doi: 10.1111/j.1365-2362.1984.tb01214.x
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- Führende Autor*innen der Med Uni Graz
- Muntean Eugen
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- Abstract:
- To study the dissociation of the two moieties of the factor-VIII complex during clotting, plasma, concentrate and serum were chromatographed on 4% agarose. In plasma and concentrate factor-VIII coagulant activity (VIII C), factor-VIII coagulant antigen (VIII C:Ag), and factor-VIII-related antigen (VIII R:Ag) eluted together in the void volume, but some VIII C:Ag eluted after the void volume. The amount of VIII C:Ag eluting after the void volume was made smaller by adding proteinase inhibitors. In serum nearly all VIII C:Ag eluted after the void volume. From factor-VIII complex immunoadsorbed by means of an antibody against VIII R:Ag no VIII C:Ag was dissociated by thrombin or by thrombin and physiologic CaCl2 concentrations. Radiolabelled human thrombin did not bind to the VIII C:Ag of immunoadsorbed factor-VIII complex. VIII C:Ag displaying VIII C was dissociated from immunoadsorbed factor-VIII complex by human brain thromboplastin or by phosphatidyl-serine. Our results suggest that VIII C:Ag and VIII R:Ag dissociate during clotting. This dissociation seems not to be mediated by thrombin, but may be mediated by phospholipids.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens - metabolism
- Blood Coagulation Tests - metabolism
- Chromatography, Gel - metabolism
- Factor VIII - immunology
- Humans - immunology
- Kinetics - immunology
- Phospholipids - physiology
- Thrombin - physiology
- von Willebrand Factor - physiology