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SHR Neuro Cancer Cardio Lipid Metab Microb

Gronlund, H; Adedoyin, J; Reininger, R; Varga, EM; Zach, M; Fredriksson, M; Kronqvist, M; Szepfalusiz, Z; Spitzauer, S; Gronneberg, R; Valenta, R; Hedlin, G; van Hage, M.
Higher immunoglobulin E antibody levels to recombinant Fel d 1 in cat-allergic children with asthma compared with rhinoconjunctivitis.
CLIN EXPERIMENT ALLERGY. 2008; 38(8): 1275-1281. Doi: 10.1111/j.1365-2222.2008.03003.x
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Co-authors Med Uni Graz: Varga Eva-Maria; Zach Maximilian
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Abstract:: BACKGROUND: Current diagnosis of allergy and asthma to cat is confirmed using cat dander extract (CDE). We have previously engineered a recombinant major cat allergen, rFel d 1, with properties identical to the natural molecule. OBJECTIVE: The aim of the study was to evaluate IgE and IgG4 antibodies to rFel d 1 among sera from cat-allergic children and adults suffering from asthma and/or rhinoconjunctivitis (RC) in populations from Sweden and Austria. METHODS: Cat-allergic children and adults from Sweden (n=27 and 31, respectively) and Austria (n=41 and 41) with RC and/or asthma were selected. Sera were tested for IgE and IgG4 antibodies to CDE and rFel d 1 by CAP, and IgE to rFel d 1 by ELISA. Healthy subjects and non-cat-allergic patients (n=75) were included as controls. RESULTS: There was a high correlation between IgE responses to rFel d 1 and CDE among the 140 patients (r(s)=0.85, P<0.001); however, measured levels to rFel d 1 were on average 30% higher (P<0.0001). Ninety-eight percent of patients and none of the controls showed IgE to rFel d 1 and there was a threefold increased risk of asthma for half of the children with the highest IgE levels [odds ratio 3.23; 95% confidence interval (CI), 1.19-8.79] by ELISA. IgE responses to rFel d 1 among children with asthma were higher (median 19.4 kU/L) compared with children with RC (median 6.6 kU/L, P<0.05) and adults with asthma (median 3.0 kU/L, P<0.01). Furthermore, children with asthma displayed higher IgG4 levels than the asthmatic adults. CONCLUSION: A single recombinant molecule, rFel d 1, is at least as sensitive for in vitro diagnostics of cat allergy as the current extract-based test. Elevated IgE antibody levels to Fel d 1 are suggested to be a risk factor for asthma in cat-allergic children.
Find related publications in this database (using NLM MeSH Indexing): Adolescent -; Adult -; Animals -; Asthma - diagnosis; Cats - immunology; Child -; Child, Preschool -; Conjunctivitis, Allergic - diagnosis; Enzyme-Linked Immunosorbent Assay -; Female -; Glycoproteins - immunology; Humans -; Immunoglobulin E - blood; Immunoglobulin G - blood; Male -; Radioallergosorbent Test -; Recombinant Proteins - immunology; Rhinitis, Allergic, Perennial - diagnosis; Sensitivity and Specificity -
Find related publications in this database (Keywords): asthma; cat; children; IgE; rFel d 1