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Selected Publication:
Höfler, H; Denk, H; Walter, GF.
Immunohistochemical demonstration of cytokeratins in endocrine cells of the human pituitary gland and in pituitary adenomas.
Virchows Arch A Pathol Anat Histopathol. 1984; 404(4):359-368
Doi: 10.1007/BF00695220
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- Co-authors Med Uni Graz
- Denk Helmut
- Walter Gerhard
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- Abstract:
- Ten non-neoplastic pituitary glands and 22 pituitary adenomas producing different hormones were studied by immunofluorescence microscopy as well as peroxidase-antiperoxidase and biotin-avidin techniques on frozen sections and formalin-fixed, paraffin-embedded material using antibodies to cytokeratin, vimentin, GFAP, neurofilament protein and different pituitary hormones. The endocrine cells in non-neoplastic pituitary glands as well as in most pituitary adenomas were cytokeratin-positive. The cytoplasmic cytokeratin distribution patterns of non-neoplastic and tumor cells were similar and typical of the type of hormone produced: GH-producing normal cells showed a paranuclear condensation of cytokeratin-reactive intermediate filaments; this accumulation was even further accentuated in GH-producing adenomas resulting in fibrous bodies (Kovacs and Horvath 1978) decorated by cytokeratin antibodies. Prolactin-producing cells showed a less intense cytoplasmic cytokeratin-specific staining with focal paranuclear accentuation in non-neoplastic as well as in neoplastic glands. ACTH-producing cells in normal pituitary glands as well as in adenomas exhibited a strong and more uniform cytoplasmic cytokeratin staining. The cytokeratin reactivity in glycoprotein hormone-producing cells of non-neoplastic tissue and adenomas was weak. Vimentin and GFAP reactivity was confined to agranular folliculo-stellate cells. The specific and different distribution patterns of cytokeratins in pituitary cells can, therefore, provide an (indirect) indication to the production of a specific hormone if immunocytochemistry fails to demonstrate hormone production.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenoma - metabolism
- Female - metabolism
- Histocytochemistry - metabolism
- Humans - metabolism
- Immunochemistry - metabolism
- Intermediate Filament Proteins - metabolism
- Keratins - metabolism
- Male - metabolism
- Microscopy, Fluorescence - metabolism
- Pituitary Gland - cytology
- Pituitary Neoplasms - metabolism
- Tissue Distribution - metabolism