Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz

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Wintersteiger, R; Juan, H.
Prostaglandin determination with fluorescent reagents.
PROSTAGLAND LEUK MED. 1984; 14(1): 25-40. Doi: 10.1016/0262-1746(84)90021-0
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Co-Autor*innen der Med Uni Graz: Juan Heinz
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Abstract:: Fluorescent derivatives of PGE2 as a model substance were formed with naphthyl isocyanate, anthracene isocyanate, 4-(6-methyl- benzothiazol -2-yl)phenyl isocyanate, benzocoumarin -3-carboxylic acid chloride and 4-bromomethyl-7- methoxycoumarin . The optimum conditions for derivatization with these reagents were investigated and the results were compared. Quantitative determination was performed by means of fluorodensitometric measurement of the derivatives. Using 4-bromomethyl-7- methoxycoumarin as a reagent as little as 100 pg of the PGE2 derivative can be detected.
Find related publications in this database (using NLM MeSH Indexing): Chemistry -; Dinoprostone -; Fluorescent Dyes -; Prostaglandins - analysis; Prostaglandins E - analysis; Spectrometry, Fluorescence - analysis