Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Avogaro, P; Bittolo Bon, G; Cazzolato, G; Pontoglio, E; Kostner, GM.
Distribution and concentration of apolipoprotein E in high and low density lipoproteins.
Ric Clin Lab. 1983; 13(4):431-436
Doi: 10.1007/BF02906923
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- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
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- Abstract:
- An electroimmunoassay of apolipoprotein E (Apo E) in total human plasma and in the supernatant following precipitation with sodium phosphotungstate in the presence of MgCl2 of the apolipoprotein B (Apo B)-containing lipoproteins is described. The assay is specific and sensitive enough to record also low levels of Apo E found in isolated lipoprotein fractions. In 30 normal male subjects, the method gave a total plasma Apo E concentration of 6.67 +/- 1.92 mg/dl and a HDL Apo E concentration of 2.58 +/- 0.91 mg/dl. Total Apo E is positively correlated with total plasma triglycerides. The values of Apo E in the lipoproteins obtained by ultracentrifugation were lower than the corresponding values obtained by a precipitation procedure, with a mean Apo E loss of 12.1% in the low density lipoproteins (VLDL + LDL) and of 34.3% in HDL. After ultracentrifugation, a substantial amount of Apo E was detected in the d greater than 1.210 g/ml fraction. The proposed method allows to study the Apo E concentration and distribution between low and high density lipoproteins in large samples, avoiding the problems connected with high salt concentrations and centrifugal forces.
- Find related publications in this database (using NLM MeSH Indexing)
- Apolipoproteins - blood
- Apolipoproteins E - blood
- Cholesterol - blood
- Humans - blood
- Immunoelectrophoresis - blood
- Lipoproteins, HDL - blood
- Lipoproteins, LDL - blood
- Lipoproteins, VLDL - blood
- Male - blood
- Triglycerides - blood