Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Koidl, C; Bozic, M; Hadzisejdic, I; Grahovac, M; Grahovac, B; Kranewitter, W; Marth, E; Kessler, HH.
Comparison of molecular assays for detection and typing of human papillomavirus.
Am J Obstet Gynecol. 2008; 199(2): 144.e1-144.e6.
Doi: 10.1016/j.ajog.2008.03.005
Web of Science
PubMed
FullText
FullText_MUG
- Führende Autor*innen der Med Uni Graz
- Kessler Harald
- Koidl Christoph
- Co-Autor*innen der Med Uni Graz
- Bozic Michael
- Marth Egon
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- OBJECTIVE: The objective of the study was to compare the performance of 3 different extraction instruments in conjunction with 4 different amplification and detection kits for detection and typing of human papillomavirus (HPV) deoxyribonucleic acid (DNA). STUDY DESIGN: A total of 42 cervical swabs were investigated. HPV DNA was extracted on the 3 different instruments. Each of the extracts was then amplified, and HPV DNA amplification products were detected with 4 different kits. RESULTS: In 31 samples, HPV DNA was detected by both the Amplicor HPV test and the LINEAR ARRAY HPV genotyping test in conjunction with DNA extraction on the easyMAG instrument. In another 6 samples, only low-risk types were detected with the linear array HPV genotyping test. After extraction on the easyMAG instrument, 32 samples tested positive when the PapilloCheck with the HotStarTaq DNA polymerase was used. CONCLUSION: Together with extraction on the easyMAG instrument, the Amplicor HPV test, the linear array HPV genotyping test, and the new PapilloCheck with the HotStarTaq DNA polymerase provide comparable results allowing reliable and safe HPV diagnostics in the routine laboratory. Use of alternative assays may lead to an increase of invalid and divergent HPV typing results.
- Find related publications in this database (using NLM MeSH Indexing)
- Cervix Uteri - virology
- DNA, Viral - isolation and purification
- Female -
- Humans -
- Nucleic Acid Amplification Techniques - methods
- Papillomaviridae - genetics
- Reagent Kits, Diagnostic -
- Reproducibility of Results -
- Vaginal Smears -
- Find related publications in this database (Keywords)
- DNA extraction
- human papillomavirus
- microarray
- polymerase chain reaction
- typing