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GRAIER, WF; SIMECEK, S; BOWLES, DK; STUREK, M.
HETEROGENEITY OF CAFFEINE-SENSITIVE AND BRADYKININ-SENSITIVE CA2+ STORES IN VASCULAR ENDOTHELIAL-CELLS
BIOCHEM J. 1994; 300: 637-641. Doi: 10.1042/bj3000637 [OPEN ACCESS]
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Leading authors Med Uni Graz: Graier Wolfgang
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Abstract:: The filling state of Ca2+ stores in endothelial cells regulates Ca2+ entry. The functional relationship between the major Ca2+ stores [i.e. Ins(1,4,5)P3-sensitive (= bradykinin-sensitive stores, 'BsS') and caffeine-sensitive stores] is unknown. In pig right-coronary-artery endothelial cells, caffeine failed to release Ca2+ in 68% of the cells (quiet-responders), but increased bradykinin (Bk)-induced Ca2+ release 2.5-fold. In Bk-pre-stimulated cells, caffeine increased Ca2+ release upon a second stimulation with Bk 3.2-fold. In quiet-responders caffeine alone did not affect net Ca2+ storage, whereas Bk or caffeine followed by Bk decreased the intracellular Ca2+ pool to 45% and 15%, respectively. Blockade of the endoplasmic-reticulum Ca2+ pump by thapsigargin unmasked the effect of caffeine in quiet-responders, resulting in a transient increase in intracellular free Ca2+ concentration ([Ca2+]i). In 37% of the cells caffeine alone transiently increased [Ca2+]i and depleted BsS. This study suggests a heterogeneity in functional organization of endothelial Ca2+ stores. In quiet-responders, caffeine translocates Ca2+ towards the BsS, whereas in overt-responders caffeine empties the BsS.
Find related publications in this database (using NLM MeSH Indexing): Animals -; Biological Transport - drug effects; Bradykinin - pharmacology; Caffeine - pharmacology; Calcium - metabolism; Calcium-Transporting ATPases - antagonists and inhibitors; Cell Compartmentation -; Cells, Cultured -; Endoplasmic Reticulum - metabolism; Endothelium, Vascular - metabolism; Swine -; Terpenes - pharmacology; Thapsigargin -