Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Farley, J; Dimai, HP; Stilt-Coffing, B; Farley, P; Pham, T; Mohan, S.
Calcitonin increases the concentration of insulin-like growth factors in serum-free cultures of human osteoblast-line cells.
Calcif Tissue Int. 2000; 67(3):247-254
Doi: 10.1007/s002230001112
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Dimai Hans Peter
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- Abstract:
- The current studies were intended to determine whether the anabolic effects of calcitonin (CT) on human osteoblast-line cells were (1) unique to osteosarcoma cells or also evident in osteoblast-line cells derived from normal human bone; and/or (2) associated with effects on several insulin-like growth factor (IGF) system components. Preliminary studies identified several osteoblastic cell lines, derived from normal human bone, which showed calcitonindependent increases in cell proliferation, alkaline phosphatase activity, and/or (45)Ca uptake (P < 0.05-P < 0.001). Two of these cell lines-(human vertebrae) HBV-155 and HBV-163-were included with the human osteosarcoma cell line, SaOS-2, in most of our subsequent studies of calcitonin effects on selected IGF system components: IGF-II, IGF-I, and IGF binding proteins -3, -4, and -5. The results of those studies revealed that a 48 hour exposure to salmon CT caused a dose-dependent (0.03-3 mU/ml) increase in the net extracellular level of IGF-II (r = 0.96, P < 0.01) in serum-free cultures of SaOS-2 cells, with a maximal 60% increase at the highest tested dose (P < 0.02). Similar effects were seen with HBV-163 cells (r = 0.90, P < 0.01) and HBV-155 cells (r = 0.55, P < 0.02). The effect of calcitonin on the extracellular level of IGF-II was biphasic with respect to time: it decreased at 6 hours (P < 0.005 and P < 0.001, for SaOS-2 cells and HBV-163 cells, respectively) and increased at 24 hours (P < 0.02 and P < 0.05). These calcitonin-dependent increases in the extracellular level of IGF-II were associated with parallel increases in IGF-I (P < 0.005 for SaOS-2 cells and P < 0.03 for HBV-163 cells), but calcitonin did not affect the extracellular level of transforming growth factor (TGF)-beta. The calcitonin-dependent changes in IGF-II were not associated with changes in the extracellular levels of IGF binding proteins -3, -4, or -5. Finally, our studies showed that two other members of the CT superfamily-CT gene-related peptide and amylin-did not mimic the effect of CT to increase the extracellular level of IGF-II. Together, these data demonstrate that human osteoblast-line cells derived from normal human bone can respond to CT, and that those responses can include CT dose- and time-dependent increases in the extracellular levels of IGF-I and IGF-II.
- Find related publications in this database (using NLM MeSH Indexing)
- Alkaline Phosphatase - metabolism
- Calcitonin - pharmacology
- Calcium - metabolism
- Culture Media, Serum-Free - metabolism
- DNA - biosynthesis
- Dose-Response Relationship, Drug - biosynthesis
- Enzyme-Linked Immunosorbent Assay - biosynthesis
- Humans - biosynthesis
- Insulin-Like Growth Factor Binding Proteins - drug effects
- Insulin-Like Growth Factor I - drug effects
- Insulin-Like Growth Factor II - drug effects
- Osteoblasts - cytology
- Osteosarcoma - metabolism
- Thymidine - metabolism
- Transforming Growth Factor beta - metabolism
- Tumor Cells, Cultured - metabolism