Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Majumder, S; Tamilarasan, KP; Kolluru, GK; Muley, A; Nair, CM; Omanakuttan, A; Murty, KV; Chatterjee, S.
Activated pericyte attenuates endothelial functions: nitric oxide-cGMP rescues activated pericyte-associated endothelial dysfunctions.
Biochem Cell Biol. 2007; 85(6):709-720
Doi: 10.1139/o07-140
Web of Science
PubMed
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- Co-Autor*innen der Med Uni Graz
- Kuppusamy Palaniappan Tamilarasan
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- Abstract:
- Hepatic stellate cells are liver-specific pericytes and exist in close proximity with endothelial cells. The activation of liver pericytes is intrinsic to liver pathogenesis, and leads to endothelial dysfunction, including the low bioavailability of nitric oxide (NO). However, the role of nitric oxide in pericyte-endothelium cross-talk has not yet been elucidated. This work examines the cellular mechanism of action of NO in pericyte-mediated endothelial dysfunction. We used in vitro coculture and conditioned medium systems to study the effects of activated liver pericytes on endothelial function, and an egg yolk vascular bed model was used to study the effects of activated pericytes on angiogenesis. This study also demonstrates that activated pericytes attenuate the migration, proliferation, permeability, and NO production of endothelial cells. Our results demonstrate that activated pericytes restrict angiogenesis in egg yolk vascular bed models, and NO supplementation recovers 70% of the inhibition. Our results also demonstrate that supplementation with NO, sildenafil citrate (phosphodiesterase inhibitor), and 8-bromo-cGMP (cGMP analog) partially recovers activated-pericyte-mediated endothelium dysfunction. We conclude that NO-cGMP alleviates activated-pericyte-associated endothelial dysfunction, including angiogenesis, in a cGMP-dependent manner.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Cell Line -
- Cell Membrane Permeability - drug effects
- Cell Movement - drug effects
- Cell Proliferation - drug effects
- Cell Surface Extensions - drug effects
- Chickens - drug effects
- Culture Media, Conditioned - pharmacology
- Cyclic GMP - metabolism
- Dose-Response Relationship, Drug - metabolism
- Endothelial Cells - cytology
- Enzyme Inhibitors - pharmacology
- Guanylate Cyclase - antagonists and inhibitors
- Hepatocytes - cytology
- Humans - cytology
- Hydrolysis - drug effects
- Neovascularization, Physiologic - drug effects
- Nitric Oxide - biosynthesis
- Pericytes - cytology
- Solubility - drug effects
- Wound Healing - drug effects
- Find related publications in this database (Keywords)
- hepatic stellate cells
- endothelial cells
- nitric oxide
- cGMP