Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Bos, ES; van der Doelen, AA; Denissen, AE; Goverde, BC; Magnani, HN; Kostner, GM.
A novel assay for the quantitative determination of lipoprotein-X (LP-X) in serum.
CLIN CHIM ACTA. 1983; 127(3): 383-390.
Doi: 10.1016/0009-8981(83)90165-1
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- Co-Autor*innen der Med Uni Graz
- Kostner Gerhard
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- Abstract:
- A new, fast and simple quantitative LP-X assay is described. The method selectively removes apoB-containing lipoproteins by precipitation with specific antibodies. To the supernatant, a precipitation system for LP-X, viz. SDS and MgCl2 in final concentrations of 0.5 g/l and 0.2 mol/l respectively, is added and the turbidity measured at 360 nm. The assay is linear from 0.2 g/l-5.0 g/l and shows a high precision (inter-assay CV of less than 3%). The test correlates favourably with other quantitative LP-X assays, but has the particular advantage that it can be automated and that the time required for one series is only 2 h.
- Find related publications in this database (using NLM MeSH Indexing)
- Antibodies -
- Electrophoresis, Polyacrylamide Gel -
- Humans -
- Immunochemistry -
- Lipoprotein-X - blood