Medizinische Universität Graz - Research portal
Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Koller, M; Bona, R; Chiellini, E; Fernandes, EG; Horvat, P; Kutschera, C; Hesse, P; Braunegg, G.
Polyhydroxyalkanoate production from whey by Pseudomonas hydrogenovora.
Bioresour Technol. 2008; 99(11):4854-4863
Doi: 10.1016/j.biortech.2007.09.049
Web of Science
PubMed
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- Co-authors Med Uni Graz
- Kutschera Christoph
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- Abstract:
- Whey permeate from dairy industry was hydrolyzed enzymatically to cleave its main carbon source, lactose, to glucose and galactose. The hydrolysis products were chosen as carbon sources for the production of poly-3-hydroxybutyric acid (PHB) by Pseudomonas hydrogenovora. In shaking flask experiments, the utilization of whey permeate as a cheap substrate was compared to the utilization of pure glucose and galactose for bacterial growth under balanced conditions as well as for the production of PHB under nitrogen limitation. After determination of the inhibition constant Ki for sodium valerate on biomass production (Ki=1.84 g/l), the biosynthesis of PHA co-polyesters containing 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) units from hydrolyzed whey permeate and valerate was investigated. The application of hydrolyzed whey permeate turned out to be advantageous compared with the utilization of pure sugars. Therefore, fermentation under controlled conditions in a bioreactor was performed with hydrolyzed whey permeate to obtain detailed kinetic data (maximum specific growth rate, mu max=0.291/h, maximum polymer concentration, 1.27 g/l PHB), values for molecular mass distribution (weight average molecular weight Mw=353.5 kDa, polydispersity index PDI=3.8) and thermo analytical data. The fermentation was repeated with co-feeding of valerate (maximum specific growth rate, mu(max)=0.201/h, maximum polymer concentration, 1.44 g/l poly-(3HB-co-21%-3HV), weight average molecular weight M(w)=299.2 kDa, polydispersity index PDI=4.3).
- Find related publications in this database (using NLM MeSH Indexing)
- 3-Hydroxybutyric Acid - metabolism
- Bioreactors -
- Fermentation - drug effects
- Galactose - pharmacology
- Glucose - pharmacology
- Hydrolysis - drug effects
- Industrial Waste -
- Ketoglutaric Acids - metabolism
- Polyesters - isolation and purification
- Polyhydroxyalkanoates - metabolism
- Pseudomonas - cytology
- Valerates - metabolism
- Find related publications in this database (Keywords)
- polyhydroxyalkanoates
- polymer characterization
- Pseudomonas hydrogenovora
- valeric acid
- whey permeate