Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Hoppe, U; Hoppe, EM; Peskar, BM; Peskar, BA.
Radioimmunoassay for leukotriene E4. Use for determination of total sulfidopeptide-leukotriene release from rat gastric mucosa.
FEBS Lett. 1986; 208(1):26-30
Doi: 10.1016/0014-5793(86)81524-1
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- Co-Autor*innen der Med Uni Graz
- Peskar Bernhard
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- Abstract:
- A conjugate of leukotriene (LT) E4 and bovine serum albumin (BSA) was prepared by covalently linking the free amino group of the hapten to the protein using dimethyl pimelindiimidate (DMP) as coupling reagent. Anti-LTE4 antibodies were raised in rabbits immunized with the conjugate. Binding of [3H]LTE4 to the antibodies is inhibited by 50% with 0.63 ng LTE4, while the relative cross-reaction of LTC4 and LTD4 is 46.3% and 12.6%, respectively. Using the radioimmunoassay release of sulfidopeptide-LT (SP-LT) from rat gastric mucosa incubated in vitro was determined after quantitative enzymatic conversion of SP-LT to LTE4. It could be demonstrated that this method is suitable for determination of SP-LT in biological material.
- Find related publications in this database (using NLM MeSH Indexing)
- Amino Acids, Sulfur - metabolism
- Animals - metabolism
- Gastric Mucosa - metabolism
- Leukotriene E4 - metabolism
- Male - metabolism
- Nordihydroguaiaretic Acid - pharmacology
- Radioimmunoassay - pharmacology
- Rats - pharmacology
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- SRS-A - analogs and derivatives