Medizinische Universität Graz - Research portal
Selected Publication:
Aehringhaus, U; Wölbling, RH; König, W; Patrono, C; Peskar, BM; Peskar, BA.
Release of leukotriene C4 from human polymorphonuclear leucocytes as determined by radioimmunoassay.
FEBS Lett. 1982; 146(1):111-114
Doi: 10.1016/0014-5793(82)80715-1
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-authors Med Uni Graz
- Peskar Bernhard
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Rabbits were immunized with a conjugate of leukotriene (LT) C4 and bovine serum albumin prepared by coupling the single free amino group of the hapten to the protein using gluteraldehyde. Binding of [3H]LTC4 to the antibodies obtained is inhibited by 50% with 1.5 ng LTC4. The relative cross-reaction of LTD4 is 16% and of LTC4-methyl ester 3.6%. The validity of the radioimmunoassay was demonstrated by comparison with bioassay using the isolated guinea pig ileum. Using the radioimmunoassay it could be shown that endogenous LTC4 is released in a dose-dependent manner by human polymorphonuclear leucocytes stimulated with the divalent cation ionophore A23187.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Calcimycin - pharmacology
- Granulocytes - metabolism
- Humans - metabolism
- Neutrophils - metabolism
- Rabbits - metabolism
- Radioimmunoassay - methods
- SRS-A - blood