Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Jürgens, G; Hermann, A; Aktuna, D; Petek, W.
Dissociation-enhanced lanthanide fluorescence immunoassay of lipoprotein(a) in serum.
Clin Chem. 1992; 38(6):853-859
Doi: 10.1093/clinchem/38.6.853
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- Jürgens Günther
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- Abstract:
- Lipoprotein(a), a human serum lipoprotein structurally related to low-density lipoprotein (LDL), contains in addition to apolipoprotein B (apo B) apolipoprotein(a) [apo(a)], a glycoprotein with a strong homology to plasminogen. Lp(a) is a risk factor for coronary heart disease and ischemic cerebrovascular disease. Several immunological techniques are used to quantify Lp(a) in human serum, including radioimmunoassays, rocket immunoelectrophoresis, and enzyme-linked immunosorbent assays. Only the last method is suitable for large-scale clinical studies. We describe another solid-phase immunoassay, based on the dissociation-enhanced lanthanide fluorescence system Delfia (Wallac Oy), and outline the technical details. A polyclonal antiserum directed against Lp(a) was used as the capture antibody. Two kinds of detection antibodies were applied, a polyclonal antiserum against apo B and the polyclonal antiserum against Lp(a). The results agree excellently with the values estimated by rocket immunoelectrophoresis. This assay is easily established, measures Lp(a) in a wide concentration range, and is suitable for screening large populations.
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- Enzyme-Linked Immunosorbent Assay -
- Europium -
- Fluorometry -
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- Immunoassay - methods
- Immunoelectrophoresis - methods
- Lipoprotein(a) - methods
- Lipoproteins - blood
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- Find related publications in this database (Keywords)
- Apolipoproteins
- Screening