Medizinische Universität Graz - Research portal
Selected Publication:
Saria, A; Lembeck, F; Skofitsch, G.
Determination of capsaicin in tissues and separation of capsaicin analogues by high-performance liquid chromatography.
J CHROMATOGR. 1981; 208(1): 41-46.
Doi: 10.1016/S0021-9673(00)87957-1
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- Co-authors Med Uni Graz
- Lembeck Fred
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- Abstract:
- Capsaicin, the pungent principle of chili peppers, and six analogues were characterized by reversed-phase high-performance liquid chromatography using isocratic methanol--water mixtures and UV detection. A highly sensitive method for the quantitative determination of capsaicin in animal tissues and blood has been developed. Using a reversed-phase column and acetonitrile--water mixtures for elution, 3 ng of capsaicin could be measured by fluorimetric detection at an excitation wavelength of 270 nm and an emission wavelength of 330 nm. Acetone extraction of tissue resulted in a recovery of approximately 90%. Extraction and determination of capsaicin in rat brain, spinal cord, liver and blood after i.v. administration is given as an example.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Brain Chemistry -
- Capsaicin - blood
- Chromatography, High Pressure Liquid - methods
- Fatty Acids, Unsaturated - blood
- Rats - blood
- Spinal Cord - analysis