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SHR Neuro Cancer Cardio Lipid Metab Microb
Kockskamper, J; Seidlmayer, L; Walther, S; Hellenkamp, K; Maier, LS; Pieske, B.
Endothelin-1 enhances nuclear Ca2+ transients in atrial myocytes through Ins(1,4,5)P3-dependent Ca2+ release from perinuclear Ca2+ stores.
J Cell Sci. 2008; 121(Pt 2):186-195
Doi: 10.1242/jcs.021386
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- Leading authors Med Uni Graz
- Kockskämper Jens
- Co-authors Med Uni Graz
- Pieske Burkert Mathias
- Walther Stefanie
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- Abstract:
- Nuclear Ca2+ plays a key role in the regulation of gene expression. Inositol (1,4,5)-trisphosphate [Ins(1,4,5)P3)] might be an important regulator of nuclear Ca2+ but its contribution to nuclear Ca2+ signalling in adult cardiomyocytes remains elusive. We tested the hypothesis that endothelin-1 enhances nuclear Ca2+ concentration transients (CaTs) in rabbit atrial myocytes through Ins(1,4,5)P3-induced Ca(2+) release from perinuclear stores. Cytoplasmic and nuclear CaTs were measured simultaneously in electrically stimulated atrial myocytes using confocal Ca2+ imaging. Nuclear CaTs were significantly slower than cytoplasmic CaTs, indicative of compartmentalisation of intracellular Ca2+ signalling. Endothelin-1 elicited a preferential (10 nM) or a selective (0.1 nM) increase in nuclear versus cytoplasmic CaTs. This effect was abolished by inhibition of endothelin-1 receptors, phospholipase C and Ins(1,4,5)P3 receptors. Fractional Ca2+ release from the sarcoplasmic reticulum and perinuclear stores was increased by endothelin-1 at an otherwise unaltered Ca2+ load. Comparable increases of cytoplasmic CaTs induced by beta-adrenoceptor stimulation or elevation of extracellular Ca2+ could not mimic the endothelin-1 effects on nuclear CaTs, suggesting that endothelin-1 specifically modulates nuclear Ca2+ signalling. Thus, endothelin-1 enhances nuclear CaTs in atrial myocytes by increasing fractional Ca2+ release from perinuclear stores. This effect is mediated by the coupling of endothelin receptor A to PLC-Ins(1,4,5)P3 signalling and might contribute to excitation-transcription coupling.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Calcium - metabolism
- Calcium Signaling -
- Cell Nucleus - metabolism
- Cytoplasm - metabolism
- Endothelin-1 - biosynthesis
- Heart Atria - metabolism
- Inositol 1,4,5-Trisphosphate - metabolism
- Inositol 1,4,5-Trisphosphate Receptors - metabolism
- Kinetics -
- Microscopy, Confocal -
- Models, Biological -
- Muscle Cells - metabolism
- Rabbits -
- Sarcoplasmic Reticulum - metabolism
- Find related publications in this database (Keywords)
- endothelin
- inositol (1
- )-trisphosphate
- calcium
- nucleus
- myocyte