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Selected Publication:
SHR Neuro Cancer Cardio Lipid Metab Microb
Dubsky, P; Hayden, H; Sachet, M; Bachleitner-Hofmann, T; Hassler, M; Pfragner, R; Gnant, M; Stift, A; Friedl, J.
Allogeneic tumor lysate can serve as both antigen source and protein supplementation for dendritic cell culture.
Cancer Immunol Immunother. 2008; 57(6): 859-870.
Doi: 10.1007/s00262-007-0422-0
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- Co-authors Med Uni Graz
- Pfragner Roswitha
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- Abstract:
- BACKGROUND: Recent preclinical and clinical evidence suggests the use of allogeneic tumor as a source of antigen for DC-based immunotherapy against cancer. We hypothesized that addition of allogeneic tumor lysate to monocyte-derived DC culture could serve a dual purpose: (1) antigen source and (2) protein supplementation of DC culture media. Protein supplementation whether of known origin (human serum/plasma, fetal bovine serum, human serum albumin) or undeclared origin ("serum-free" media) is a source of variability and bias. We addressed the question whether protein supplementation can be omitted in the presence of allogeneic tumor lysate. MATERIALS AND METHODS: Human DC cultured in the presence of lysate from medullary thyroid carcinoma (MTC) cell line SHER-I (TuLy-DC) and DC pulsed with the same lysate but cultured in the presence of FBS (FBS-DC) were assessed for morphology, phenotype, maturation and functional properties. RESULTS: In comparison of FBS-DC/TuLy-DC no significant differences in morphology, phenotype and maturation could be detected. Both culture conditions produced CD1a(high), CD14(low) DC with high expression of costimulatory molecules and CD83 upon stimulation. TuLy-DC gave significantly better yields and produced more IL12p70. DC showed high (allo)stimulatory capacity toward T-cells. TuLy-DC induced more intracellular IFNgamma in CD8+T-cells of vaccinated MTC patients. Both types of DC induced killing of SHER-I after short in vitro restimulation. Tumor lysate from SHER-I can substitute for further protein supplementation in DC culture. Allogeneic tumor lysates should be taken into consideration as both source of antigen and protein supplementation in monocyte-derived DC culture.
- Find related publications in this database (using NLM MeSH Indexing)
- Antigens, CD3 - biosynthesis
- Antigens, Neoplasm - chemistry
- Carcinoma - immunology
- Cell Culture Techniques - methods
- Cell Line, Tumor -
- Culture Media, Serum-Free - pharmacology
- Dendritic Cells - cytology
- Humans -
- Immunotherapy - methods
- Interleukin-10 - metabolism
- Interleukin-4 - metabolism
- Neoplasms - immunology
- Proteins - chemistry
- Thyroid Neoplasms - immunology
- Transplantation, Homologous - methods
- Find related publications in this database (Keywords)
- dendritic cells
- immunotherapy
- tumor lysate
- medullary thyroid carcinoma
- DC culture