Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Földes-Papp, Z; Birch-Hirschfeld, E; Eickhoff, H; Baumann, G; Peng, WG; Biber, T; Seydel, R; Kleinschmidt, AK; Seliger, H.
Fractals for multicyclic synthesis conditions of biopolymers. Examples of oligonucleotide synthesis measured by high-performance capillary electrophoresis and ion-exchange high-performance liquid chromatography.
J Chromatogr A. 1996; 739(1-2):431-447
Doi: 10.1016/0021-9673(96)00060-X
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- Földes-Papp Zeno
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- Abstract:
- We have developed models of patterns for nucleotide chain growth. These patterns are measurable by high-performance capillary electrophoresis and ion-exchange high-performance liquid chromatography in crude products of solid-phase synthesized 30mer and 65mer oligodeoxyribonucleotide target sequences N. We introduce mathematical methods for finding characteristic values d(o) and p(o) for constant chemical modes of growth as well as d and p for non-constant chemical modes of growth (d = probability of propagation, p = probability of termination). These methods are employed by presenting the accompanying computer software developed by us in C code, Mathematica R languages, and Fortran. Characteristic values of the parameters d, p, and the target nucleotide length N describe the complete composition of the crude product. From this we have developed the relation 2 - [N/(N - 1)]/Da, measurable(N,d) as a universal quantitative measure for multicyclic synthesis conditions (D, fractal dimension and similarity exponent, respectively). We use this mathematical treatment to compare the efficiency of oligodeoxyribonucleotide syntheses of different target length N on polymer support materials. Further, we analyze selected syntheses of short and long oligodeoxyribonucleotides as well as single-stranded DNA sequences by well-known empirical autocorrelation, fast Fourier transformation, and embedding dimension techniques.
- Find related publications in this database (using NLM MeSH Indexing)
- Base Sequence -
- Chromatography, High Pressure Liquid - methods
- Chromatography, Ion Exchange - methods
- Electrophoresis, Capillary - methods
- Fractals - methods
- Mathematics - methods
- Models, Chemical - methods
- Molecular Sequence Data - methods
- Oligonucleotides - chemical synthesis
- Software - chemical synthesis
- Find related publications in this database (Keywords)
- oligonucleotide synthesis, chemical
- nucleotide sequence variability
- gene assembly
- fractal dimension
- data analysis
- polymerization processes
- enzymatic nucleotide growth
- oligodeoxyribonucleotides
- oligonucleotides
- oligoribonucleotides
- DNA