Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Földes-Papp, Z; Rigler, R.
Quantitative two-color fluorescence cross-correlation spectroscopy in the analysis of polymerase chain reaction.
Biol Chem. 2001; 382(3): 473-478.
Doi: 10.1515/BC.2001.057
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- Führende Autor*innen der Med Uni Graz
- Földes-Papp Zeno
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- Abstract:
- We present results of an approach in which low-density labeled DNA itself provides an amplification of the cross-correlated fluorescent signal in the two-color cross-correlation function. Tetramethylrhodamine-4-dUTP and Cy5-dCTP are incorporated by polymerase chain reaction at multiple positions of the same 217 bp target DNA. We call this novel approach the 'two-color FCS signal amplification'. The signal amplification is an example for interactions of two ligands with different colors at multiple positions of the same target.
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- Find related publications in this database (Keywords)
- absolute molecule number
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