Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Wagner, SN; Ockenfels, HM; Wagner, C; Soyer, HP; Goos, M.
Differential expression of tissue inhibitor of metalloproteinases-2 by cutaneous squamous and basal cell carcinomas.
J INVEST DERMATOL 1996 106: 321-326.
Doi: 10.1111%2F1523-1747.ep12342979
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- Co-Autor*innen der Med Uni Graz
- Soyer Hans Peter
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- Abstract:
- Tumor cell invasion and metastasis are considered to represent a multistep process leading to the degradation of the extracellular matrix by proteolytic enzymes. The functional activity of matrix metalloproteinases (MMPs) is controlled by tissue inhibitor of metalloproteinases-2 (TIMP-2), which has been shown to inhibit tumor cell invasion and metastasis in vitro and in vivo. To assess the role of TIMP-2 in skin-derived epithelial tumors, we have analyzed the expression of TIMP-2 mRNA in primary tissue samples from human cutaneous basal (BCC) and squamous cell carcinoma (SCC) for a correlation with their different invasive and metastatic potential. Comparative quantitative analysis of TIMP-2 mRNA levels by Northern blot hybridization demonstrated significantly higher TIMP-2 tissue levels in BCC than in SCC, indicating an inverse correlation between TIMP-2 expression and the metastatic capacity of these tumors in vivo. By in situ hybridization, tumor stromal cells were identified as the principal source of TIMP-2 mRNA in both BCC and SCC. A comparable distribution has been reported previously for several matrix metalloproteinases in cutaneous BCC and SCC, indicating co-localization of metalloproteinases with their respective inhibitor. These results may suggest that TIMP-2 substantially contributes to the biological behavior of epithelium-derived skin tumors by significantly inhibiting tumor cell metastasis.
- Find related publications in this database (using NLM MeSH Indexing)
- Carcinoma, Basal Cell - chemistry
- Carcinoma, Squamous Cell - chemistry
- Gene Expression - chemistry
- Humans - chemistry
- Protease Inhibitors - metabolism
- Proteins - genetics
- RNA, Messenger - analysis
- Skin - chemistry
- Skin Neoplasms - chemistry
- Tissue Inhibitor of Metalloproteinase-2 - chemistry
- Transcription, Genetic - chemistry
- Find related publications in this database (Keywords)
- Northern Blot Hybridization
- In Situ Hybridization
- Invasion
- Metastasis