Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
COTTEN, M; WAGNER, E; ZATLOUKAL, K; PHILLIPS, S; CURIEL, DT; BIRNSTIEL, ML.
High-efficiency receptor-mediated delivery of small and large (48 kilobase gene constructs using the endosome-disruption activity of defective or chemically inactivated adenovirus particles.
PROC NAT ACAD SCI USA. 1992; 89(13): 6094-6098.
Doi: 10.1073/pnas.89.13.6094
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- Co-Autor*innen der Med Uni Graz
- Zatloukal Kurt
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- Abstract:
- One limit to successful receptor-mediated gene delivery is the exit of the endocytosed material from the endosome. We demonstrate here the delivery of marker genes to tissue culture cells using a modification of the receptor-mediated gene delivery technique that exploits the endosomolytic activity of defective adenovirus particles. In particular, greater than 90% of the transfected-cell population is found to express a beta-galactosidase gene, and, most importantly, this high level of expression can be obtained with psoralen-inactivated virus particles. Furthermore, because the delivered gene is not carried within the genome of the adenovirus particle, the size constraints are relieved, and we can, therefore, show the delivery of a 48-kilobase cosmid DNA molecule.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenoviruses, Human - genetics
- Cells, Cultured - genetics
- Defective Viruses - genetics
- Endocytosis - genetics
- Endosomes - physiology
- Gene Expression - physiology
- Genetic Vectors - physiology
- Hela Cells - physiology
- Humans - physiology
- Molecular Weight - physiology
- Receptors, Cell Surface - physiology
- Transfection - physiology
- Ultraviolet Rays - physiology
- Virus Replication - physiology
- Find related publications in this database (Keywords)
- GENE THERAPY
- ENDOCYTOSIS
- TRANSFERRIN
- ASIALOGLYCOPROTEIN
- COSMID