Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
WAGNER, E; ZATLOUKAL, K; COTTEN, M; KIRLAPPOS, H; MECHTLER, K; CURIEL, DT; BIRNSTIEL, ML.
Coupling of adenovirus to transferrin-polylysine/DNA complexes greatly enhances receptor-mediated gene delivery and expression of transfected genes.
Proc Natl Acad Sci U S A. 1992; 89(13):6099-6103
Doi: 10.1073/pnas.89.13.6099
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- Co-Autor*innen der Med Uni Graz
- Zatloukal Kurt
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- Abstract:
- We are developing efficient methods for gene transfer into tissue culture cells. We have previously shown that coupling of a chimeric adenovirus with polylysine allowed the construction of an adenovirus-polylysine-reporter-gene complex that transferred the transporter gene with great efficiency into HeLa cells. We have now explored simpler, biochemical means for coupling adenovirus to DNA/polylysine complexes and show that such complexes yield virtually 100% transfection in tissue culture cell lines. In these methods adenovirus is coupled to polylysine, either enzymatically through the action of transglutaminase or biochemically by biotinylating adenovirus and streptavidinylating the polylysine moiety. Combination complexes containing DNA, adenovirus-polylysine, and transferrin-polylysine have the capacity to transfer the reporter gene into adenovirus-receptor- and/or transferrin-receptor-rich cells.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenoviruses, Human - genetics
- Animals - genetics
- Cells, Cultured - genetics
- Chloroquine - pharmacology
- DNA - administration and dosage
- Endocytosis - administration and dosage
- Gene Expression - administration and dosage
- Hela Cells - administration and dosage
- Humans - administration and dosage
- Liver - cytology
- Mice - cytology
- Polylysine - chemistry
- Receptors, Transferrin - physiology
- Receptors, Virus - physiology
- Transfection - physiology
- Transferrin - metabolism
- Find related publications in this database (Keywords)
- GENE THERAPY
- ENDOCYTOSIS
- ENDOSOME
- GENE TRANSFER
- DNA TRANSFECTION