Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
PREISEGGER, KH; ZATLOUKAL, K; SPUREJ, G; DENK, H.
Changes of cytokeratin filament organization in human and murine Mallory body-containing livers as revealed by a panel of monoclonal antibodies.
Liver. 1991; 11(5):300-309
Doi: 10.1111/j.1600-0676.1991.tb00533.x
Web of Science
PubMed
FullText
FullText_MUG
Google Scholar
- Co-Autor*innen der Med Uni Graz
- Zatloukal Kurt
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- Mallory bodies (MBs) are characteristics morphologic features of alcoholic hepatitis and can be produced in mouse hepatocytes by chronic griseofulvin (GF) intoxication. The formation of MBs, which share some immunological, biochemical, and ultrastructural features with cytokeratin (CK) filaments of normal liver, is accompanied by derangement and even loss of the CK cytoskeleton of hepatocytes ("empty cells") as revealed by immunofluorescence microscopy. To clarify whether this diminution or lack of CK-related staining of MB-containing hepatocytes was due to loss of CK filaments or changes in antigenicity or accessibility of antigenic determinants immunohistochemical studies using a battery of monoclonal and polyclonal CK antibodies were performed. It could be shown that all these antibodies directed against different CK polypeptide components and antigenic determinants of CKs revealed a highly reduced or even undetectable cytoplasmic CK meshwork in most cells with fully developed large MBs. In the light of our present knowledge of the organization of CK intermediate filaments, these results indicate that the phenomenon of the "empty cells" reflects a diminution of CK meshwork rather than altered antigenic determinants.
- Find related publications in this database (using NLM MeSH Indexing)
- Animals -
- Antibodies, Monoclonal -
- Bile Ducts, Intrahepatic - chemistry
- Desmin - analysis
- Electrophoresis, Polyacrylamide Gel - analysis
- Griseofulvin - toxicity
- Hepatitis, Alcoholic - metabolism
- Humans - metabolism
- Immunoblotting - metabolism
- Inclusion Bodies - chemistry
- Intermediate Filaments - chemistry
- Keratins - analysis
- Liver - chemistry
- Male - chemistry
- Mice - chemistry
- Microscopy, Fluorescence - chemistry
- Vimentin - analysis
- Find related publications in this database (Keywords)
- ALCOHOLIC HEPATITIS
- CYTOSKELETON
- GRISEOFULVIN
- INTERMEDIATE FILAMENTS
- MALLORY BODIES