Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
MAASS, G; SCHWEIGHOFFER, T; BERGER, M; SCHMIDT, W; HERBST, E; ZATLOUKAL, K; BUSCHLE, M.
Tumor vaccines: effects and fate of IL-2 transfected murine melanoma cells in vivo.
Int J Immunopharmacol. 1995; 17(2):65-73
Doi: 10.1016/0192-0561(94)00085-3
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- Co-Autor*innen der Med Uni Graz
- Zatloukal Kurt
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- Abstract:
- We have previously demonstrated the general usefulness of the adenovirus-enhanced transferrinfection (AVET) in the generation of IL-2 producing tumor vaccines. By optimizing different parameters of the transfection protocol we were able to transform the poorly immunogenic M-3 mouse melanoma cell line into a potent immunogen. A long-lasting immunity was demonstrated after administration of the IL-2 releasing vaccine, since immunized animals successfully rejected native M-3 melanoma cells even after a period of more than 6 months. We also demonstrated that in vivo administration of such a vaccine is safe since transmission of the transfected IL-2 gene in host organs was not detected. IL-2 production ceased 2 days after injection because the engineered cells were destroyed. However, RT-PCR analysis of the site of vaccine injection suggests that IL-2 exerts its effects not only directly but also by inducing a set of other immunomodulator cytokines in situ that are probably indispensable in inducing a host response. We conclude that AVET of IL-2 into tumor cells is a safe and efficient method for the generation of tumor vaccines.
- Find related publications in this database (using NLM MeSH Indexing)
- Adenoviridae - genetics
- Animals - genetics
- Female - genetics
- Interleukin-2 - biosynthesis
- Male - biosynthesis
- Melanoma, Experimental - genetics
- Mice - genetics
- Mice, Inbred DBA - genetics
- Time Factors - genetics
- Transfection - methods
- Tumor Cells, Cultured - methods
- Vaccination - methods
- Vaccines, Attenuated - pharmacology
- Find related publications in this database (Keywords)
- TUMOR VACCINE
- M-3 MURINE MELANOMA MODEL
- INTERLEUKIN-2
- ADENOVIRUS-ENHANCED TRANSFERRINFECTION