Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Strunk, D; Rappersberger, K; Egger, C; Strobl, H; Krömer, E; Elbe, A; Maurer, D; Stingl, G.
Generation of human dendritic cells/Langerhans cells from circulating CD34+ hematopoietic progenitor cells.
Blood. 1996; 87(4):1292-1302
Doi: 10.1182/blood.V87.4.1292.bloodjournal8741292
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- Führende Autor*innen der Med Uni Graz
- Strunk Dirk
- Co-Autor*innen der Med Uni Graz
- Strobl Herbert
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- Abstract:
- Human Langerhans cells (LC) are CD1a+ dendritic cells (DC) that function as potent antigen-presenting cells for primary and secondary immune responses. Limitations in DC/LC numbers, imposed by difficult and tedious isolation procedures, have so far precluded their use as immunogens in the generation and/or augmentation of host responses against various pathogens. Therefore, we have developed a procedure for the generation of human DC/LC from CD34+ hematopoietic progenitor cells (HPC) isolated (mean: 0.7 x 10(6)/ buffy coat and 2.6 x 10(6)/leukapheresis product) and purified ( > 95%) from the peripheral blood of healthy adults. In vitro stimulation of these cells with granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor (TNF)-alpha led to their vigorous proliferation and differentiation resulting in the emergence of CD45+/CD68+/CD3-/CD19-/CD56- leukocytes some of which (mean: 12%) express CD1a and exhibit anti-CD4 and anti-major histocompatibility complex (MHC) class II reactivity. These CD1a- leukocytes include (1) LC as evidenced by the presence of Birbeck granules (BG), (2) CD14+ monocytes, and (3) Birbeck granule-negative cells with a dendritic morphology. Addition of interleukin (IL)-4 to the cytokine cocktail interfered with the development of monocytes and led to a reduction in the overall yield but, on the other hand, resulted in an increased percentage of CD1a+ cells (mean: 24%) among all cells generated. In vitro generated CD1a+, but not CD1a- HPC-derived cells are potent stimulators of the primary mixed leukocyte reaction and, as such, promising candidates for vaccination purposes.
- Find related publications in this database (using NLM MeSH Indexing)
- Adult -
- Antigens, CD1 - analysis
- Antigens, CD34 - analysis
- Blood Cells - cytology
- Cell Division - cytology
- Cell Separation - cytology
- Cells, Cultured - cytology
- Dendritic Cells - cytology
- Granulocyte-Macrophage Colony-Stimulating Factor - pharmacology
- Hematopoietic Stem Cells - cytology
- Humans - cytology
- Interleukin-4 - pharmacology
- Langerhans Cells - cytology
- Lymphocyte Culture Test, Mixed - cytology
- Lymphocyte Depletion - cytology
- Tumor Necrosis Factor-alpha - pharmacology