Medizinische Universität Graz - Research portal
Selected Publication:
Lamprecht, G; Kraushofer, T; Stoschitzky, K; Lindner, W.
Enantioselective analysis of (R)- and (S)-atenolol in urine samples by a high-performance liquid chromatography column-switching setup.
J Chromatogr B Biomed Sci Appl. 2000; 740(2): 219-226.
Doi: 10.1016/S0378-4347(00)00080-3
Web of Science
PubMed
FullText
FullText_MUG
- Co-authors Med Uni Graz
- Stoschitzky Kurt
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- An HPLC column-switching method for the enantioselective determination of (R,S)-atenolol in human urine was developed and validated. Diluted urine samples were injected onto a LiChrospher ADS restricted access column and atenolol was separated from most of the matrix components using 0.01 M Tris buffer. The atenolol peak was sharpened by a step gradient of 30% acetonitrile and the atenolol-containing fraction was switched onto an enantioselective column. Separation of the atenolol enantiomers was carried out on a Chirobiotic T (Teicoplanin) column using acetonitrile-methanol-acetic acid-triethylamine (55:45:0.3:0.2, v/v/v/v) as eluent. Detection of the effluent was performed by fluorescence measurement. Several experiments were carried out to suppress the high blank reading, which was efficiently achieved using Tris buffer in the first dimension. For the enantioselective analysis of (R)- and (S)-atenolol in plasma under the same conditions the sample capacity of the ADS column is considerably lower.
- Find related publications in this database (using NLM MeSH Indexing)
- Adrenergic beta-Antagonists - blood
- Atenolol - blood
- Calibration - blood
- Chromatography, High Pressure Liquid - blood
- Humans - blood
- Reproducibility of Results - blood
- Spectrometry, Fluorescence - blood
- Stereoisomerism - blood
- Find related publications in this database (Keywords)
- atenolol
- restricted access
- column switching
- enantiomer separation