Medizinische Universität Graz - Research portal
Selected Publication:
Schaffer, P; Ahammer, H; Müller, W; Koidl, B; Windisch, H.
Di-4-ANEPPS causes photodynamic damage to isolated cardiomyocytes.
PFLUGERS ARCH, EUR J PHYSIOL. 1994; 426(6): 548-551.
Doi: 10.1007/BF00378533
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PubMed
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- Leading authors Med Uni Graz
- Schaffer Peter
- Co-authors Med Uni Graz
- Ahammer Helmut
- Koidl Bernd
- Müller Wolfram
- Windisch Herbert
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- Abstract:
- Action potential recordings from isolated guinea pig ventricular cells in the whole-cell recording mode were used to study the toxic and photodynamic properties of the voltage-sensitive fluorescent dye di-4-ANEPPS. Staining of the cardiomyocytes with di-4-ANEPPS (30 or 60 microM; 10 min) did not alter the action potential shape. When the stained cells were illuminated (1W/cm2) severe effects on the action potential were observed. There was a prolongation of the action potential duration, occurrence of early afterdepolarizations, reduction of the membrane resting potential and eventually inexcitability. Addition of the antioxidant catalase (100 IU/ml) to the extracellular solution delayed the onset of these effects, suggesting that reactive-oxygen-intermediates take part in di-4-ANEPPS induced photodynamic damage. Since di-4-ANEPPS is a very important tool for optical membrane potential recordings in heart tissue and single cardiomyocytes catalase might be useful in suppressing photodynamic damage during optical potential recordings.
- Find related publications in this database (using NLM MeSH Indexing)
- Action Potentials - drug effects
- Animals - drug effects
- Catalase - pharmacology
- Electrophysiology - pharmacology
- Guinea Pigs - pharmacology
- Heart - drug effects
- Heart Ventricles - cytology
- Light - cytology
- Membrane Potentials - drug effects
- Myocardium - cytology
- Pyridinium Compounds - toxicity
- Find related publications in this database (Keywords)
- Voltage-Sensitive Dye
- Di-4-Anepps
- Photodynamic Damage
- Ventricular Cells
- Catalase