Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
Traebert, M; Trebess, I; Erlenkamp, S; Hüser, J; Kockskämper, J; Glitsch, HG; Hartung, C; Welzel, P.
High affinity regulation of cardiac Cl- and Ca2+ conductances by (13R)-spiroforskolin.
Naunyn Schmiedebergs Arch Pharmacol. 1998; 358(5):538-546
Doi: 10.1007/PL00005290
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- Co-Autor*innen der Med Uni Graz
- Kockskämper Jens
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- Abstract:
- The effects of a new forskolin derivative, (13R)-spiroforskolin, on the ventricular cAMP-activated chloride current (I(Cl(cAMP))) and the atrial L-type calcium current (I(Ca,L)) were measured by means of whole-cell recording from isolated guinea-pig cardiac myocytes at 30 degrees C and 20-22 degrees C, respectively. In contrast to forskolin, the derivative contains a tetrahydrofuran rather than a tetrahydropyran moiety. (13R)-spiroforskolin activated I(Cl(CAMP)) in 58% of the ventricular myocytes studied. The concentration required for the half maximal effect (EC50 value) amounted to 9.6x10(-11) M and was lower than the EC50 value for forskolin (2.4x10(-8) M). (13R)-spiroforskolin evoked a smaller maximal I(Cl(cAMP)) amplitude than forskolin. The rundown of the (13R)-spiroforskolin-activated I(Cl(cAMP)) was faster than that of the forskolin-induced current. Neither forskolin nor (13R)-spiroforskolin in maximally effective concentrations increased I(Cl(cAMP)) in cells containing high concentrations of cAMP. Furthermore, as an activator of atrial I(Ca,L) (13R)-spiroforskolin displayed a smaller activation and a lower EC50 value (5.8x10(-10) M) than forskolin (EC50 value: 3.7x10(-7) M). The effect of (13R)-spiroforskolin was observed in only 30% of the atrial cells studied. None of the drugs exerted a stimulatory effect in atrial cells containing a high [cAMP]. The washout of the drug effect was significantly faster in (13R)-spiroforskolin- than in forskolin-treated atrial myocytes. We conclude that (13R)-spiroforskolin as a forskolin derivative displays unique characteristics. It is a more potent but less efficacious activator of cardiac ionic conductances than the parent compound. The results suggest that (13R)-spiroforskolin, like forskolin, most probably exerts its effects via stimulation of the adenylyl cyclase.
- Find related publications in this database (using NLM MeSH Indexing)
- 8-Bromo Cyclic Adenosine Monophosphate - pharmacology
- Action Potentials - drug effects
- Animals - drug effects
- Atrial Function - drug effects
- Calcium Channels - drug effects
- Chloride Channels - drug effects
- Cricetinae - drug effects
- Cyclic AMP - metabolism
- Dose-Response Relationship, Drug - metabolism
- Electric Stimulation - metabolism
- Forskolin - analogs and derivatives
- Heart Atria - cytology
- Heart Ventricles - cytology
- Membrane Potentials - drug effects
- Myocardium - cytology
- Patch-Clamp Techniques - cytology
- Sarcolemma - drug effects
- Spiro Compounds - pharmacology
- Time Factors - pharmacology
- Find related publications in this database (Keywords)
- (13R)-spiroforskolin
- forskolin
- cAMP-activated chloride current
- L-type calcium current
- whole-cell recording
- guinea-pig cardiomyocytes