Medizinische Universität Graz Austria/Österreich - Forschungsportal - Medical University of Graz
Gewählte Publikation:
SHR Neuro Krebs Kardio Lipid Stoffw Microb
Lamprecht, G; Stoschitzky, K.
Determination of carvedilol in human plasma by high-performance liquid chromatography applying on-line deproteination and column switching
CHROMATOGRAPHIA 2004 59: 551-554.
Doi: 10.1365/s10337-004-0275-z
Web of Science
FullText
FullText_MUG
- Co-Autor*innen der Med Uni Graz
- Stoschitzky Kurt
- Altmetrics:
- Dimensions Citations:
- Plum Analytics:
- Scite (citation analytics):
- Abstract:
- An HPLC-column switching method has been developed and validated for the determination of carvedilol in human plasma. 20 muL plasma was injected onto a Brownlee C8 column and the high molecular weight proteins were eluted isocratically. Carvedilol was separated from the majority of the components by a linear acetonitrile gradient. The fraction containing carvedilol was switched on-line onto a LiChrospher RP 18 column and separated isocratically from accompanying compounds applying 0.1 M triethylamine-methanol-acetonitrile = 17: 18:19 (v/v/v). The influence of pH and temperature on retention was investigated on both columns. No interference from other plasma components was observed. Reproducibility of the complete method was better than 4% and the limit of quantitation was 0.8 ng mL(-1).
- Find related publications in this database (Keywords)
- column liquid chromatography
- column switching
- on-line deproteination
- carvedilol